Protective effects of EPA and DHA on lipopolysaccharide-stimulated rat mesangial cells
- VernacularTitle:EPA、DHA对脂多糖刺激大鼠系膜细胞的保护作用
- Author:
Xiaojing HU
;
Wenjing GENG
;
Bo JIAO
;
Fange LIU
- Publication Type:Journal Article
- Keywords:
Eicosapentaenoic acid;
Docosahexaenoic acid;
Mesangial cells;
Transforming growth factor beta;
Monocyte chemoattractant protein-1
- From:
Chinese Journal of Pathophysiology
2010;26(3):513-517
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of EPA and DHA on oxidative stress of lipopolysaccharide-stimulated rat mesangial cells. METHODS: The glomerular mesangial cells (GMCs) were stimulated by lipopolysaccharide (LPS) and incubated with EPA (10 μmol/L or 100 μmol/L) and DHA (10 μmol/L or 100 μmol/L) for 24 h, 48 h and 72 h. The activity of SOD, GSH-Px and the level of MDA was measured. The protein and mRNA expressions of MCP-1 and TGF-β_1 were detected by immunocytochemistry and real-time PCR method, respectively. RESULTS: The activities of SOD and GSH-Px were decreased and the concentration of MDA was increased when stimulated with LPS. EPA and DHA increased the activities of SOD and GSH-Px and decreased the concentration of MDA significantly. Meanwhile, the protein and mRNA expressions of MCP-1 and TGF-β_1 stimulated by LPS were decreased. DHA was more effective than EPA at the same concentration. CONCLUSION: EPA and DHA enhance the activities of antioxidant enzymes, decrease the concentration of MDA and inhibit the expression of TGF-β_1 and MCP-1, suggesting that the protective effect of EPA and DHA on kidney is related to the antioxidation and the inhibition of TGF-β_1 and MCP-1 expression.
