Klotho: a protein molecule interacting with FPC protein
- VernacularTitle:Klotho: 与FPC蛋白相互作用的蛋白质分子
- Author:
Liping SUN
;
Xinzhou ZHANG
;
Chaofeng HU
- Publication Type:Journal Article
- Keywords:
Autosomal recessive polycystic kidney disease;
Fibrocystin/ polycystin;
Yeast two hybrid system
- From:
Chinese Journal of Pathophysiology
2010;26(3):518-523
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of fibrocystin/polycystin (FPC) in autosomal recessive polycystic kidney disease (ARPKD) development by means of screening the protein interaction using yeast two-hybrid approach. METHODS: The constructed pGBKT7-FPC was used as the bait to screen the pre-transformed human fetal kidney cDNA expression library by yeast two-hybrid assay to obtain the host cell protein which interacted with C-terminal region of FPC. The sequence transformation screening experiment was applied to confirm the protein interactions in yeast. RESULTS: After yeast mating and co-transformation screening analysis, Klotho (KL) was selected from the host cells and the interaction between KL and FPC was further confirmed. CONCLUSION: C-terminal region of FPC can interact with KL, which probably provide the approach for further studying the role and biochemistry mechanism of FPC protein in ARPKD.
