Antigen screening and identification of Helicobacter pylori outer membrane proteins from murine infection model
- VernacularTitle:利用小鼠感染模型筛选与鉴定幽门螺杆菌外膜蛋白抗原
- Author:
Haoxia TAO
;
Peng WANG
;
Shengling YUAN
;
Dewen ZHAN
;
Lingchun WANG
;
Yanchun WANG
;
Chunjie LIU
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
Animal disease model;
Two-dimensional gel electrophoresis;
Immunology;
Antigen screening
- From:
Chinese Journal of Immunology
2010;26(3):195-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen antigen of Helicobacter pylori outer membrane proteins by murine infection model.Methods:Parallel two-dimensional gel electrophoresis (2D) of outer membrane proteins extracted from Helicobacter pylori strain SS1 was performed.Western blot of a duplicate 2D gel hybridized with serum from H.pylori-infected murine was employed.Immunogenic H.pylori proteins identified in this way were digested in gel by trypsin and the mass of generated peptides were measured by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).The data obtained from peptide mass finger-printing (PMF) were searched using the internet available database.Results:32 proteins were identified and they are in good agreement with typical protective antigens which reacted with serum from H.pylori-infected patients.Conclusion:The results suggest that murine model of H.pylori may be valid to screen antigens for human vaccination and the proteins identified in this paper are valuable for the selection of H.pylori protective antigens as well.
