The change of periphery and central lymphocyte subsets at the crest-time of experimental autoimmune encephalomyelitis mice
- VernacularTitle:实验性自身免疫性脑脊髓炎小鼠发病高峰期外周及中枢淋巴细胞亚群的变化
- Author:
Nana XI
;
Rongyuan ZHENG
;
Xiaofeng SHANG
;
Tan WANG
;
Jin Lü
;
De XU
;
Zhenggang WU
;
Guoqian CHEN
- Publication Type:Journal Article
- Keywords:
Experimental autoimmune encephalomyelitis;
Lymphocyte subset;
Multiple sclerosis;
T lymphocyte;
B lymphocyte
- From:
Chinese Journal of Immunology
2010;26(3):236-240
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the change of periphery and centra lymphocyte subsets at the crest-time of MOG_(35-55) induced EAE disease in mice,and to explore the alteration of cellular immunity and humoral immunity in the invasion process in EAE.Methods:MOG_(35-55) was used to establish EAE model in femina C57BL/6 mice.The behavioral changes and the histological scores were recorded after the mice were immuned .The changes of CD3~+CD4~+,CD3~+CD8~+,CD4~+CD25~+ and B220~+ on periphery and centra lymphocytes in spleen,brain and spinal cord were analyzed by flow cytometry.Results:The CD3~+CD4~+,CD3~+CD8~+,CD4~+CD25~+ and B220~+ lymphocytes were detected in the brain and spinal cord of EAE group mice,but they were not detected in CFA control group.The CD3~+CD4~+ and CD3+CD8+lymphocytes in the spleen of EAE crest-time group were lower than those in CFA control group(P＜0.05).The B220~+ lymphocytes were obviously higher than in the CFA control group (P＜0.01).And CD4~+CD25~+ lymphocytes were slight higher than the CFA control group.Conclusion:At the crest-time during EAE,the CD3~+CD4~+,CD3~+CD8~+lymphocytes of spleen reduced obviously,B220~+ lymphocytes increased markedly,and the CD4~+CD25~+ lymphocytes just have the increasing trend.It indicates that cellular immunity and humoral immunity coregulated the patho-process at the crest-time of EAE,T lymphocytes and B lymphocytes all played important roles in the pathogenesy of EAE.
