Down regulation of HMGA2 expression changes malignant phenotypes the malignant phenotype of human osteosarcoma U2OS cells
- VernacularTitle:下调HMGA2基因表达对改善骨肉瘤U2OS细胞恶性表型的实验研究
- Author:
Shanshan QU
;
Ronggui LI
;
Haiying ZHANG
;
Yang WANG
;
Yanfen SHI
;
Hui Lü
;
Yulin LI
- Publication Type:Journal Article
- Keywords:
HMGA 2;
shRNA;
U20S;
Proliferation;
Apoptosics
- From:
Chinese Journal of Immunology
2010;26(3):228-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The roles of HMGA2 in maintaining malignant phenotypes of the osteosarcoma U2OS cells was studied to explore the possibilities for it to be developed as a target for gene therapy.Methods:U2OS cells were stably transfected with a DNA based shRNA expression vector which targeted to HMGA2.The expression of HMGA2 mRNA was proved by RT-PCR;Cell growth,migration and apoptosis were determined with CCK8,hoechst33342 staining and Boyden ventricle,respectively.The mRNA levels of Caspase 3 and Caspase 9 were determined by real time quantitative RT-PCR.Results:The transfection with shRNA expression vector significantly decreased HMGA2 mRNA levels of U2OS cells.Cell growth and migration were decreased,but apoptosis and the mRNA levels of Caspase 3 and Caspas 9 were increased following the decrease of HMGA2 mRNA.Conclusion:The abnormal expression of HMGA2 plays an important role in maintaining the malignant phenotypes of U2OS cells.Gene therapy targeted to HMGA2 could be helpful in the treatment of human osteosarcoma.
