Plant vector construction and expression of Der f1 allergen of Dermatophagoides pteronyssinu
- VernacularTitle:尘螨变应原Der f1植物表达载体的构建及表达
- Author:
Jianglong PENG
;
Yubao CUI
;
Huamin WANG
;
Ying ZHOU
;
Lina NIU
;
Jie WU
- Publication Type:Journal Article
- Keywords:
Dermatophagoides pteronyssinu;
Der f1;
Potato virus X (PVX);
Plant expression
- From:
Chinese Journal of Immunology
2010;26(3):250-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the plant expression vector of Der f1 allergen of Dermatophagoides pteronyssinu and expression in tobacco lamina.Methods:The Der f1 gene was amplified from the glycerin bacterium which contained pET28a(+)-Der f1 plasmid,cloned into the pMD 19-T plasmid,and then sequenced.The Der f1 gene was digested by ClaⅠand SalⅠ,and cloned into potato virus X (PVX) to construct plant expression vector PVX-Der f1,and then was transformed agrobacterium tumefaciens.The positive one was selected to infect tobacco lamina for expressing target protein.The protein was identified and analysed by SDS-PAGEand Western blot.Results:Digestion and sequence analysis confirmed that the plant expression vector was correct,and the SDS-PAGE and Western blot results showed that the molecular weight of the protein was about 34M_r and it could specific binding with positive serum.Conclusion:The plant expression vector of Der f1 is successfully constructed and the recombinant protein is also produced.
