Electrochemical Deoxyribonucleic Acid Biosensor Based on Multi-walled Carbon Nanotubes/Ag-TiO_2 Composite Film for Label-free Phosphinothricin Acetyltransferase Gene Detection by Electrochemical Impedance Spectroscopy
10.3724/SP.J.1096.2010.00301
- VernacularTitle:多壁碳纳米管/纳米Ag-TiO_2膜DNA电化学生物传感器
- Author:
Na ZHOU
;
Tao YANG
;
Kui JIAO
;
Caixia SONG
- Publication Type:Journal Article
- Keywords:
Deoxyribonucleic acid biosensor;
Multi-walled carbon nanotubes;
Silver-titanium oxide compo-site;
Phosphinothricin acetyltransferase gene;
Electrochemical impedance spectroscopy
- From:
Chinese Journal of Analytical Chemistry
2010;38(3):301-306
- CountryChina
- Language:Chinese
-
Abstract:
A highly sensitive electrochemical deoxyribonucleic acid(DNA) biosensor based on multi-walled carbon nanotubes(MWNT)/Ag-TiO_2 composite film was developed. The solution containing Ag-TiO_2-MWNT composite was casted on the carbon paste electrode surface to form a robust film, which combine the advantages of the good biocompatibility of Ag-TiO_2 naocomposite and the fine conductivity, as well as the large active surface area of carbon nanotubes. The composite could greatly improve the immobilization capacity of the probe DNA. The morphologies and electrochemistry of the nanocomposite film were investigated by scanning electron microscopy and electrochemical techniques including electrochemical impedance spectroscopy and cyclic voltammetry, respectively. DNA hybridization events were monitored by a label-free method of electrochemical impedance spectroscopy. This label-free electrochemical impedance DNA biosensor showed high sensitivity and selectivity for phosphinothricin acetyltransferase gene sequence assay. The multicomponents films also displayed a high stability during repeated regeneration and hybridization process.
