Derivatization of Oligosaccharides and Analysis by Matrix-assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry
10.3724/SP.J.1096.2010.00307
- VernacularTitle:寡糖衍生化及基质辅助激光解吸电离飞行时间质谱分析方法研究
- Author:
Huanhuan HAN
;
Yan MA
;
Lu WANG
;
Wanjun ZHANG
;
Junying WEI
;
Yangjun ZHANG
;
Xiaohong QIAN
- Publication Type:Journal Article
- Keywords:
Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry;
1-(4-Cyanophenyl)-4-piperidinecarbohydrazide;
Neutral oligosaccharides
- From:
Chinese Journal of Analytical Chemistry
2010;38(3):307-312
- CountryChina
- Language:Chinese
-
Abstract:
A derivatization method for the determination of oligosaccharides with high sensitivity by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry(MALDI-TOF-MS) based on a labeling regent 1-(4-Cyanophenyl)-4-piperidinecarbohydrazide(CPH) was proposed. The hydrazones were generated by the reaction of the reducing ends of oligosaccharides and the hydrazide of CPH. The derivatives were directly analyzed by MALDI-TOF-MS. Reaction conditions such as reaction temperature, concentration of acetic acid and excess ratio of CPH regent were optimized. When the oligosaccharides reacted with 100 time excess of CPH at 100 ℃ and in 0.125% acetic acid (V/V), the yield was highest. The results showed the derivatization with CPH increased the sensitivity of MS detection and this method was suitable for the analysis of oligosaccharides′ compositions.
