The protective action of chromomycin on dopaminergic neurons
- VernacularTitle:色霉素对多巴胺能神经元的保护作用
- Author:
Wenya WANG
;
Mingtao LI
;
Jinjun RAO
;
Xiaonan ZHU
- Publication Type:Journal Article
- Keywords:
chromomycin;
MPP~+;
dopaminergic neurons;
cell culture;
Parkinson's disease;
Tau
- From:
Chinese Pharmacological Bulletin
2010;26(4):522-526
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of chromomycin on MPP~+-induced dopaminergic neuronal apoptosis.Methods An apoptosis model induced by 1-methyl-4-phenylpridnium ion(MPP~+)was established in cultured fetal mesencephalic neurons in vitro.Dopaminergic neuronal apoptosis was detected by Hoechst 33258 staining and phospho-Tau levels were detected by immunofluorescence.Results 10 μmol·L~(-1) MPP~+ hyperphosphorylated Tau at Ser396 and induced dopaminergic neuronal apoptosis.Chromomycin increased the number of TH-positive cells via inhibiting Tau phosphorylation.Conclusion These results indicate thatchromomycin inhibits Tau phosphorylation at Ser396 and therefore protects dopaminergic mesencephalic neurons from apoptosis induced by MPP+,which suggests that Chromomycin may be used to cure neurodegenerative disorders such as Parkinson's disease in clinic.
