The study of resveratrol by modulating SATA3 on acute myeloblastic leukemia
- VernacularTitle:白藜芦醇调节STAT3抗急性髓性白血病作用的研究
- Author:
Wei WANG
;
Tan LI
;
Hong CHEN
;
Xiaoqin GUO
;
Xiulong NIU
- Publication Type:Journal Article
- Keywords:
resveratrol;
cell cycle;
apoptosis;
leukemia;
signal transdaction;
STAT3
- From:
Chinese Pharmacological Bulletin
2010;26(3):346-352
- CountryChina
- Language:Chinese
-
Abstract:
Aim To discuss the pathway of resveratrol for anti-acute myeloblastic leukemia.Methods Human acute myeloblastic leukemia cell Kasumi-1 was applied in this study.After different doses of Res treatment for different time, methyl thiazolyl tetrazolium(MTT)colorimetry was used to detect the cell proliferation.Furthermore, apoptosis and cell cycle were detected by flow cytometry.Western blot and reverse transcription polymerase chain reaction(RT-PCR)were used to detect the expression levels of Bcl-2 and Bax for mRNA and protein.The activity of signal transducers and activators of transcription 3(STAT3)was detected by immunoblotting and Luciferase reporter gene assay.On the other hand, leukemia-bearing mice models were made to investigate the live situation, survival time and the activation of STAT3 by Western blot and immunohistochemical method.Results Resveratrol could suppress the proliferation of Kasumi-1, and induce cell cycle arrest and apoptosis.At the same time, the ratios of Bcl-2/Bax and phosphor-signal transducers and activators of transcription 3(p-STAT3)were decreased significantly.In vivo, resveratrol could prolong the life span of Kasumi-1-bearing mice and attenuate the activity of STAT3 in a dose-dependent manner.Conclusions Resveratrol is a natural occurring chemotherapeutic agent present in Chinese Herbals.The regulatory effect on signaling pathways involved in STAT3 by resveratrol may be associated with its anti-leukemia effect.
