Effect of total flavonids of Litsea coreana Leve on cytokines production and immunity of peritoneal macrophage from collagen-induced arthritis
- VernacularTitle:豹皮樟总黄酮对胶原性关节炎大鼠腹腔巨噬细胞产生细胞因子及其免疫功能的影响
- Author:
Qian ZHOU
;
Jun LI
;
Tingyu WANG
;
Xiaohua WANG
- Publication Type:Journal Article
- Keywords:
Litsea coreana Leve;
flavonoid;
arthritis;
macrophage;
immunity;
phagocytosis
- From:
Chinese Pharmacological Bulletin
2010;26(3):353-358
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of total flavonids of Litsea coreana Leve(TFLC)on cytokines production and immunity of peritoneal macrophage(PMΦ)from collagen-induced arthritis(CIA).Methods CIA was induced with Collagen II and Feund's complete adjuvant.The effect of TFLC on CIA was evaluated by body weight, hind paw swelling and histopathological changes.Interleukin-1(IL-1)in PMΦ of CIA rats was measured by mouse thymocyte proliferation method.Interleukin-2(IL-2)in splenocytes of CIA rats was determined by mouse spleen lymphocyte proliferation reaction.Tumor necrosis factor-α(TNF-α)in the supernatant of PMΦ was measured by radioimmunoassay(RIA).And the mRNA expression of TNF-α was detected by RT-PCR.Phagocytosis of PMΦ was measured by neutral red method.Results TFLC(50, 100, 200 mg·kg~(-1))singnificantly inhibited the paw swelling and increased body weight of CIA rats.The pathological damage of knee joint was reversed by TFLC.TFLC(0.05, 0.5, 5 mg·L~(-1))markedly reduced IL-1 and IL-2 in CIA rats.Meanwhile, TFLC singnificantly reduced TNF-α level and inhibited the expression of TNF-α mRNA in PMΦ.Conclusion TFLC has marked therapeutic effect on CIA rats, which is related to reducing the phagocytosis and inhibiting the secretion and expression of cytokines.
