Effect of diltiazem on cytokines expression in mononuclearcells induced by concanavalin A
- VernacularTitle:地尔硫卓对刀豆凝集素诱导单个核细胞细胞因子表达的影响
- Author:
Ying LIU
;
Xiang CHENG
;
Yuhua LIAO
- Publication Type:Journal Article
- Keywords:
diltiazem;
cytokines;
mononuclearcell;
concanavalin A;
T lymphocyte;
calcium
- From:
Chinese Pharmacological Bulletin
2010;26(3):376-378
- CountryChina
- Language:Chinese
-
Abstract:
Aim To research into the effect of diltiazem on cytokines expression in mononuclearcells induced by concanavalin A.Methods Ficoll density gradient centrifugation was used to separate the mononuclearcells from rat's spleen.There were 3 groups including control, Con A, diltiazem-Con A group in the study.The cytokines expressions in supernatant were detected by ELISA.Results Compared with control, IL-10, TNF-α, IL-6 were increased significantly in Con A group with low level IL-1β and non level TGF-β_1.But in diltiazem-Con A group, IL-10, TNF-α, IL-6 were decreased significantly compared with Con A group.Conclusion Diltiazem inhibits IL-10, TNF-α, IL-6 expressions in mononuclearcells induced by Con A.
