Determination of Terbutaline Sulfate and Its Combination with β_2 Adrenergic Receptor in Erythrocyte Membrane by High Performance Liquid Chromatography
10.3724/SP.J.1096.2010.00377
- VernacularTitle:高效液相色谱法测定硫酸特布他林与红细胞膜表面β_2肾上腺素受体结合量
- Author:
Xinhui JIANG
;
Qixin ZHOU
- Publication Type:Journal Article
- Keywords:
Terbutaline sulfate;
Propranolol hydrochloride;
Erythrocyte membranes;
β_2-Adrenergic receptor;
High performance liquid chromatography
- From:
Chinese Journal of Analytical Chemistry
2010;38(3):377-380
- CountryChina
- Language:Chinese
-
Abstract:
A high performance liquid chromatographic method with hypersil C_(18) column, 0.020 mol/L potassium dihydrogen phosphate solution(which contains 0.25% triethylamine, pH 5.1)-methanol(20∶ 80, V/V) as mobile phase and fluorescence detection with 280 nm as excitation wavelength and 320 nm as emission wavelength has been developed for the determination of terbutaline sulfate and propranolol hydrochloride in Erythrocyte suspensions. Erythrocyte surface receptor oscillating reacted 1 h with terbutaline in 37 ℃ water bath. Free terbutaline and propranolol were separated from the membrane receptor-binding complex through 1500 r/min centrifugal in 5 min. The linear range was 0.010-3.000 mg/L in Alsever's solutions and the correlation coefficients were 0.9999 and 0.9998. The relative standard deviations(RSDs) of terbutaline and propranolol were 0.8% and 1.2%, respectively. The limits of detection(LODs) of both were 1.00 and 3.00 mg/L. This simple, safety and sensitive method was suitable for the determination of terbutaline and propranolol in erythrocyte suspensions and for the study of receptor-ligand binding.
