Probucol inhibits proliferation of rat aortic smooth muscle cells stimulated with oxidized low-density lipoprotein
- VernacularTitle:Probucol抑制氧化低密度脂蛋白诱导的大鼠主动脉平滑肌细胞增殖
- Author:
Lin SHENG
;
Lijuan SHAO
;
Lin HAO
;
Dongling XU
;
Xinglei WANG
;
Bo JIAO
;
Qixing PAN
- Publication Type:Journal Article
- Keywords:
Probucol;
Oxidized low-density lipoproteins;
Thioredoxin-1;
Heme oxygenase-1;
Extracellular signal-regulated kinases;
Mitogen-activated protein kinase phosphatase-1;
Smooth muscle cells
- From:
Chinese Journal of Pathophysiology
2010;26(3):440-445
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the relationships between antiproliferative mechanisms of probucol and protein expressions of signaling molecules ERK1/2, MKP-1, HO-1 and Trx-1 in rat aortic smooth muscle cells (RASMCs) stimulated with ox-LDL. METHODS: The effects of probucol on cell cycle, cell proliferation and the expressions of ERK1/2, MKP-1, HO-1 and Trx-1 in the presence of ox-LDL were observed by means of MTT test, FCM and Western blotting. RESULTS: (1) Probucol significantly inhibited the proliferation of RASMCs stimulated with ox-LDL. A value in 100 μmol/L probucol+35 mg/L ox-LDL group was reduced by 34.9% as compared to ox-LDL group (P<0.01). (2) Probucol protected against ox-LDL-induced RASMCs proliferation through inducing cell growth arrest at G_0/G_1 phase and cell apoptosis. (3) ox-LDL increased the expression of p-ERK1/2 by 34.7% (P<0.01) and decreased MKP-1 by 60.0% (P<0.01), respectively, as compared to control. Probucol attenuated the increase in ox-LDL-stimulated p-ERK1/2 level by 15.7%, but increased MKP-1 expression by 2 times (P<0.01). (4)ox-LDL at concentration of 35 mg/L decreased the intracellular Trx-1 expression by 28.9% (P<0.05), and slightly increased the level of HO-1 expression as compared to control (P<0.05). Probucol enhanced the expression of Trx-1 by 91.6% (P<0.01) and HO-1 by 31.9% (P<0.01), respectively as compared to ox-LDL group. CONCLUSION: Probucol inhibits ox-LDL-stimulated the proliferation of RASMCs through increases in MKP-1/HO-1 expression, suppression of cell cycle progression and induction of cell apoptosis.
