Expression of genes in MAPKs pathway in development of neural tube defects induced by hyperthermia
- VernacularTitle:MAPKs信号转导蛋白在高温致神经管畸形中的表达
- Author:
Wen LIU
;
Jinlong MA
;
Wanrun LIN
;
Xingxin XU
;
Yanli GAO
;
Li LIANG
- Publication Type:Journal Article
- Keywords:
Hyperthermia;
Neural tube defects;
Mitogen-activated protein kinase kinases;
Golden hamster;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2010;26(3):472-476
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the expression and the role of ERK1/2 and JNK1/2 of MAPKs pathways in the development of neural tube defects induced by hyperthermia. METHODS: The animal models of golden hamster were produced by hyperthermia. The expression of ERK1/2 and JNK1/2, and levels of their phosphorylation were measured by Western blotting in control group and hyperthermia group. RESULTS: p-ERK1/2 steadily expressed in each control group, and the expression of p-ERK1/2 significantly decreased, which was different from that in the corresponding control group (P<0.05). The activity of p-JNK1/2 increased in hyperthermia group and the amount of p-JNK1/2 increased as compared to control group. The peak appeared at 16 h after exposed to hyperthermia (P<0.05). CONCLUSION: Hyperthermia, which induces a decrease in p-ERK1/2 expression and increases the expression of p-JNK1/2 of MAPKs pathway, results in the unbalance of cell proliferation and apoptosis, and induces neural tube defects.
