14-3-3 tau regulates invasiveness of BeWo cells via ERK1/2 pathway
- VernacularTitle:14-3-3 tau蛋白通过ERK信号通路调控BeWo细胞的侵袭功能
- Author:
Yan CHENG
;
Rong HU
;
Hong JIN
;
Kaidong MA
;
Xiaotian LI
- Publication Type:Journal Article
- Keywords:
Protein 14-3-3 tau;
Trophocytes;
BeWo cells;
Invasiveness;
ERK1/2 signal pathway
- From:
Chinese Journal of Pathophysiology
2010;26(3):563-567
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the potential role of 14-3-3 tau in trophoblast cells on invasiveness. METHODS: 14-3-3 tau expression was detected in first-trimester villi, deciduas and human trophoblastic cell line (BeWo) by immunohistochemistry. Small interference RNA (siRNA) targeting 14-3-3 tau was transfected into BeWo cells. The effects of down-regulated 14-3-3 tau on invasion of human trophoblasts cell line BeWo were examined by matrigel invasion assay, and the transcription, translation of E-cadherin and snail were estimated by RT-PCR or Western blotting. U0126 was used to detect the extracellular-signal related kinase 1/2 (ERK1/2) function on down-regulation of 14-3-3 tau induced cell invasion. RESULTS: 14-3-3 tau was detected in the invasive trophoblastic cells in the first trimester villi and that invaded to the deciduas. BeWo cells also expressed 14-3-3 tau. Down-regulation of 14-3-3 tau increased the invasive cell-number of BeWo, as well as the expression of snail, and inhibited E-cadherin. U0126 inhibited the enhanced invasiveness in these cells induced by the down-regulation of 14-3-3 tau. CONCLUSION: 14-3-3 tau may regulate the invasiveness of human trophoblastic cells through ERK1/2 signaling pathway.
