Construction of tissue-engineered skin using basic fibroblast growth factor gene transfected bone marrow mesenchymal stem cells combined with small intestinal submucosa
10.3969/j.issn.1673-8225.2010.07.008
- VernacularTitle:转染碱性成纤维细胞生长因子骨髓间充质干细胞复合小肠黏膜下层构建的组织工程皮肤
- Author:
Shaoyun WANG
;
Hao YANG
;
Xiaosong ZHU
;
Qing LI
;
Shihe LI
;
Jian DONG
;
Di WU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(7):1174-1178
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Studies demonstrated that small intestinal submucosa (SIS) had no immunogenicity, which can not lead to rejection following transplantation, thus, this is an ideal skin substitutes for natural skin.OBJECTIVE: Basic fibroblast growth factor (bFGF) gene was transfected into bone marrow mesenchymal stem cells (BMSCs),and combined the cells with SIS to construct tissue-engineered skin.METHODS: BMSCs were obtained from Japanese big-earad rabbits, and in vitro cultured. Then the subculturad BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry. In addition, the result of transfecting BMSCs with pCDNA-bFGF vector was measured by Western blot, and the structure of tissue-engineered skin was observed.RESULTS AND CONCLUSION: After passaged, BMSCs were grown quickly with Iong-fusiform shape. The cells were positive expressed CD90 and CD44, but negative expressed CD45. bFGF had been transfected into BMSCs, and stable expressed. The transfected BMSCs grew well in SIS. By this method, tissue-engineered skin can be constructed in vitro.
