Hemangioblastic characteristics of human adipose tissue-derived adult stem cells in vivo
10.3969/j.issn.1000-4718.2010.04.007
- VernacularTitle:成人脂肪源间充质干细胞在小鼠体内具有血液血管干细胞特性
- Author:
Huizhen MA
;
Ning LI
;
Yongping SONG
;
Ying CAO
;
Ziming DONG
;
Baijun FANG
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cells;
Endothelial cells;
Adipose tissue
- From:
Chinese Journal of Pathophysiology
2010;26(4):656-661
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether Flk1~+ CD31~- CD34~- cells isolated from human adult adipose tissue have characteristics of hemangioblasts in vivo. METHODS: After sublethally irradiated (300cGy) with a caesium source, the female non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice were injected with human adipose tissue-derived Flk1~+ CD31~- CD34~- cells (10~5 cells per mouse) via tail vain with 0.4 mL Roswell Park Memorial Institute medium (RPMI-1640). The control mice received the same volume of RPMI-1640 medium. All mice were killed 2 months after transplantation for further study. The differentiation potential of Flk1~+ CD31~- CD34~- cells was assessed in bone marrow and gastrointestinal tract by the methods of flow cytometry, RT-PCR, FISH, and triple-color immunofluorescence. RESULTS: Flk1~+ CD31~- CD34~- human adipose tissue-derived adult stem cells differentiated into endothelial cells and hematopoietic cells at the single-cell level in vivo. CONCLUSION: Human adult adipose tissue-derived Flk1~+ CD31~- CD34~- cells bear characteristics of hemangioblast in vivo and may have potential application for the treatment of hematopoietic and vascular diseases.
