Inhibitory effect of EGCG on proliferation and HIF-1α/VEGF expression in cell line HepG2
10.3969/j.issn.1000-4718.2010.04.018
- VernacularTitle:EGCG抑制肝癌细胞株HepG2增殖及HIF-1α/VEGF的表达
- Author:
Zhicheng ZHAO
;
Li ZHUANG
;
Changku JIA
;
Shusen ZHENG
- Publication Type:Journal Article
- Keywords:
Epigallocatechin gallate;
Liver neoplasms;
Hypoxia inducible factor-1;
Vascular endothelial growth factors
- From:
Chinese Journal of Pathophysiology
2010;26(4):713-720
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the molecular mechanism of EGCG on inhibiting the growth of hepatic carcinoma. METHODS: The proliferation of hepatic cell line HepG2 cultured with different doses of EGCG was studied by MTT and suspension/adherence methods. The effect of EGCG on the expression of HIF-1α/VEGF at mRNA and protein levels in vitro and in vivo was evaluated by RT-PCR and Western blotting, respectively. The inhibition of EGCG on the growth of tumor implanted into athymic nude mice was also observed. RESULTS: The proliferation of hepatic cell line HepG2 was inhibited by EGCG in a dose-dependent manner. The expression of HIF-1α/VEGF was suppressed markedly by EGCG at protein level. However, the inhibitory effect of EGCG on the mRNA expression was only observed on VEGF, not on HIF-1α. In the animal experiment, the implanted tumor growth was inhibited by 39.8%±5.1%. CONCLUSION: EGCG suppresses the hepatic carcinoma cell growth, and interrupts the HIF-1α/VEGF signaling pathway significantly, indicating a fundamental mechanism of EGCG for inhibiting tumor growth.
