Bioinformatic analysis of streptococcus mutans thrS gene and construction of homologous recombinant plasmids
10.3969/j.issn.1673-8225.2010.07.021
- VernacularTitle:变形链球菌thrS基因生物信息学分析及同源重组质粒的构建
- Author:
Xiaodi LIU
;
Jin DUAN
;
Lihong GUO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(7):1231-1234
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Streptococcus mutans (S. mutans) is generally considered to be the principal aetiological agent for dental caries, thrS gene may relate to the virulence of S. mutans involved in the adherence, acidogenicity and acidodurance.OBJECTIVE: To investigate the conservation status of the thrS gene of S. mutans and to construct the homologous recombinant plasmid.METHODS: Southern Blot was used to analyze the distdbuUon of thrS gene in S. mutans. The upstream and downstream sequences of thrS gene were cloned respectively into multiple cloning sites of suicide plasmid pFW5 to construct the recombinant plasmid,RESULTS AND CONCLUSION: The thrS gene was conserved in 6 strains of S. mutans in this test. By PCR analysis and enzyme digesting, it was confirmed that S. mutans thrS gene homologous recombinant plasmid was successfully constructed,which can be used in future research of construction of thrS -negative mutans of S. mutans strain UA159.
