Effects of Diazepam on Restraint Stress-induced Fos Expression in the Rat Brain.
- Author:
Seong Il CHO
1
;
Sung Pil LEE
;
Chang Uk LEE
;
Woo Sun KIM
;
In Ho PAIK
Author Information
1. Department of Psychiatry, Catholic University of Korea, College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Hippocampal formation;
Stress;
Diazepam;
c-fos
- MeSH:
Animals;
Brain*;
Dentate Gyrus;
Diazepam*;
Gyrus Cinguli;
Hippocampus;
Neurons;
Paraventricular Hypothalamic Nucleus;
Peritoneum;
Rats*
- From:Journal of Korean Neuropsychiatric Association
1997;36(5):927-934
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was conducted to Identify the effects of diazepam on stress-induced c-fos expression In rat brain. The rats were divided into 4 groups according to injection agents [0.9% isotonic saline 1ml, diazepam(5 mg/kg)] and restraint stress, respectively. Saline or diazepam was injected into the peritoneum of rats 10 minutes before the restraint groups were restrained in a wire chamber. Fos-immunoreactive neurons were calculated in the hippocampal area CA2, dentate gyrus, amygdalohippocampal area, paraventricular nucleus, ventral lateral geniculate nucleus, supramammillary nucleus, temporal cortex area 3, occipital cortex area 2, piriform cortex, and cingulate cortex area 3. The results were as follows: 1) In the hippocampal formation including hippocampal area CA2 and dentate gyrus, Fos expression increased significantly in the saline-injected restraint animals than in any other groups. A two-way factorial ANOVA retreated a significant erects of stress and diazepam, and a significant stress by diazepam interaction. 2) In amygdalohippocampal area, Fos expression increased significantly in the saline-injected restraint animals than in diazepam-injected animals. A two-way factorial ANOVA revealed a significant effects of stress and diazepam, but no significant Interaction of stress and diazepam. 3) In paraventricular nucleus, Fos expression increased significantly in the saline-injected restraint animals than in saline-Injected animals and diazepam-injected animals. A two-way factorial ANOVA revealed a significant effects of stress, no significant effects of diazepam, and no significant Interaction of stress and diazepam. 4) In ventral lateral geniculate nucleus, Fos expression Increased significantly in the saline-injected restraint animals than in diazepam-Infected animals and diazepam-injected restraint animals. A two-way factorial ANOVA revealed a significant effects of diazepam, no significant effects of stress, and no significant interaction of stress and diazepam. 5) In supramammillary nucleus, temporal cortex area 3, occipital cortex area 2, piriform cortex, and cingulate cortex area 3, there was no significant difference of Fos expression in each group. A two-way factorial ANOVA revealed no significant effects of stress and diazepam, and no significant interaction of stress and diazepam. These results suggest that the hippocampal formation is involved in the response to the stress and the tension reduction effect of diazepam.
