Role of miR-24 in the regulation of 3T3-L1 adipicyte differentiation and the expression of fatty acid binding protein
- VernacularTitle:MiR-24对3T3-L1脂肪细胞分化及脂肪型脂肪酸结合蛋白表达的调节作用
- Author:
Limei YAN
;
Qiuyan LIANG
;
Jinhua XU
;
Jianyong WU
;
Rongjiao WANG
;
Xiaohua YU
;
Jingou XU
;
Hesheng OU
- Publication Type:Journal Article
- Keywords:
microRNA;
3T3-L1 preadipocyte;
adipocyte fatty acid binding protein;
diabetes;
gene expression;
adipocyte diferentiation]
- From:
Chinese Pharmacological Bulletin
2010;26(4):483-487
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of microRNAs on the differentiation of 3T3-L1 adipocytes and the expression of adipo-related gene-fatty acid binding protein during the adipocyte differentiation.Methods adipo-related microRNAs during 3T3-L1 adipocyte differentiation were screened and identified by micorRNA microarray.Constructed high-expression plasmids of the adipo-related microRNAs,were transfected into the 3T3-L1 preadipocytes by lipofectamine.While the effect of adipo-related microRNAs on the course of 3T3-L1 adipocyte differentiation was observed,the protein and mRNA expression level of fatty acid binding protein(FABP4)were analyzed by Western blot and RT-PCR during 3T3-L1 adipocyte differentiation.Results The expression profiles of microRNAs have significant changed during 3T3-L1 adipocyte differentiation,in which 35 microRNAs among them down-relation,the most lowly expression is miR-24;17 microRNAs among them up-relation,the most highly expression is miR-21.MiR-24 significantly inhibited adipocyte differentiation and maturity,while miR-21 have no significant effect.MiR-24 significantly inhibited the expression of FABP4,but had no effect on the level of its mRNA;miR-21 had no effect on the expression of protein and mRNA of FABP4.Conclusion There exist adipogenic-related microRNAs during 3T3-L1 adipocyte differentiation; miR-24 play an important role in the regulation of 3T3-L1 preadipocyte differentiation into adipocyte and the(FABP4)protein expression.
