Anti-hepatoma activity of nitidine chloride and its effect on topoisomerase
- VernacularTitle:氯化两面针碱的抗肝癌活性及对DNA拓扑异构酶的影响
- Author:
Limin LIU
;
Huagang LIU
- Publication Type:Journal Article
- Keywords:
Zanthoxylum nitidum(Roxb)DC;
nitidine chloride;
hepatic carcinoma;
nude mice;
anti-tumor;
DNA topoisomerase
- From:
Chinese Pharmacological Bulletin
2010;26(4):497-500
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the anti-tumor effect of nitidine chloride(NC)on human HepG2 hepatocellular transplanted tumor in nude mice and its effect on topoisomerase.Methods The subcutaneous transplantable tumor model of human liver cancer in nude mice was established and the anti-tumor effect of NC was calculated.The effects of NC on TopoⅠ/Ⅱ mediated-pBR322 DNA relaxation were measured by using agarose gel electrophoresis.Results NC inhibited significantly the growth of hepatoma,The inhibitory rate at the dose of 2.5,5,10 mg·kg~(-1) was 12.06%,35.63% and 60.91% respectively.At the concentration of 6.25 μmol·L~(-1),NC completely inhibited the pBR322 DNA cleavage mediated by TopoⅠ;at the concentration of 25 μmol·L~(-1),NC completely inhibited the pBR322 DNA cleavage mediated by Topo Ⅱ.Conclusion Nitidine Chloride can inhibit hepatic carcinoma growth in nude mice,The anti-tumor mechanism is probably related to the inhibitory effect on Topo.
