Diallyl disulfide induces human leukemia HL-60 cells differentiation by up-regulating the expressions of p21,STAT1 and CAMTA1
- VernacularTitle:二烯丙基二硫上调p21、STAT1和CAMTA1诱导人白血病HL-60细胞分化
- Author:
Weiguo HUANG
;
Hui TAN
;
Lan YI
;
Jie HE
;
Qi SU
- Publication Type:Journal Article
- Keywords:
diallyl disulfide;
leukemia;
HL-60 cells;
differentiation;
p21;
STAT1;
CAMTA1
- From:
Chinese Pharmacological Bulletin
2010;26(4):513-516
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the molecular mechanisms of differentiation in human leukemia HL-60 cells induced by diallyl disulfide(DADS)using suppression subtractive hybridization(SSH).Methods In our privious study,the subtractive cDNA library was constructed successfully and efficiently. 30 clones were randomly analyed with restriction enzyme.The inserts of cDNAs were analyzed by restrictive enzyme EcoR I.Positive clones were sequenced and the homology of resulting cDNA sequences were analyzed through bioinformatics software Blastn.Results 18 clones contained 100～600 bp cDNA inserts.10 differantiation genes were obtained and involved in cell cycle,signal transduction,metabolism and RNA binding.And 3 of 10 genes,p21,STAT1 and CAMTA1 were up-regulated and detected by RT-PCR,the results matched with SSH.Conclusion sThere are tight correlation between the differentiation induced by DADS and three-upregulated gene:p21,STAT1 and CAMTA1.
