Role of survivin in PC12 cells against injuries induced by chemical hypoxia
- VernacularTitle:Survivin在PC12细胞对抗化学性缺氧损伤中的作用
- Author:
Jinlan MENG
;
Yanfen DONG
;
Liqiu MO
;
Chuntao YANG
;
Aiping LAN
;
Zhanli YANG
;
Peixi CHEN
;
Jianqiang FENG
- Publication Type:Journal Article
- Keywords:
cobalt chloride;
chemical hypoxia;
survivin;
heat shock protein 90;
PC12 cell;
17-allylamino-17-demethoxygeldanamycin
- From:
Chinese Pharmacological Bulletin
2010;26(4):526-530
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of survivin in PC12 cells against injuries induced by cobalt chloride(CoCl_2).Methods PC12 cells were exposed to CoCl_2 at different doses in different time to set up the chemical hypoxia induced PC12 cells injuries model.Cell viability was tested by using cell counter kit-8.Dose-effect(200～1 000 μmol·L~(-1))and time-effect(0～48 h)relationship between hypoxia induced by CoCl_2 and the expression of survivin was evaluated by western blot.Results PC12 cells viability was inhibited significantly by CoCl_2 in a dose and time dependent manners;At the concentrations from 200 to 600 μmol·L~(-1) CoCl_2 for 24 h,survivin expression was upregulated in a dose dependent manner,peaking at 600 μmol·L~(-1) CoCl_2 treatment,exceeding this concentration of CoCl_2,with dose increasing,survivin expression decreased.At the dose of CoCl_2 up to 1 000 μmol·L~(-1),survivin did not express basically;Treatment with 600 μmol·L~(-1) CoCl_2 in different time,within the range of 0～36 h,the expression of survivin enhanced in time dependent manner.But with the extension of time,survivin expression was declining; 17-allylamino-17-demethoxygeldanamycin (2 μmol·L~(-1)),an inhibitor of Hsp90,not only decreased survivin overexpression but also obviously enhanced the injuries of PC12 cells induced by CoCl_2,which didn't damage PC12 cells alone.Conclusion Upregulation of survivin expression may be one of the endogenous defense mechanisms for PC12 cells against chemical hypoxia.
