Analysis of Differentially Expressed Proteome in Urinary Exosome from Non-small Cell lung Cancer Patients
10.3724/SP.J.1096.2010.00325
- VernacularTitle:非小细胞肺癌患者尿中Exosomes蛋白质组的差异表达分析
- Author:
Yi DAI
;
Yuan ZHANG
;
Feng QIU
;
Yanyan LI
;
Zongyin QIU
- Publication Type:Journal Article
- Keywords:
Exosome;
Non-small cell lung cancer;
Sodium dodecylsulfonate-polyacrylate gel electrophoresis;
High performance liquid chromatography-chip-tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2010;38(3):325-331
- CountryChina
- Language:Chinese
-
Abstract:
Urine provides an alternative to blood plasma as a potential source of disease biomarkers. Exosomes was separated by ultracentrifuge at 200000 g in normal persons and non-small cell lung cancer (NSCLC) patients′ urine. For proteomic analysis of urinary exosome, 1D sodium dodecylsulfonate-polyacrylate gel electrophoresis(SDS-PAGE) was carries out and cut the gel 31 kDa-20 kDa bands in normal group and disease group′s. These gel blocks were subjected to in-gel trypsinization, and the extracted peptides were analyzed HPLC-CHIP-MS/MS. Approximately 24 unique proteins were identified in the UniProtKB/SWISS-PORT. The difference expression proteins were found in urinary exosome from NSCLC patients, including three fragment of the immunoglobulin kappa, two kinds of Ras related proteins, glutathione S-transferase A2, serum amyloid P-component precursor and phosphatidylethanolamine-binding protein 1.
