Differential Proteomic Analysis of Metastasis-associated Proteins in Mice Melanoma
10.3969/j.issn.1000-8179.2010.05.002
- VernacularTitle:小鼠黑色素瘤转移相关蛋白的差异蛋白质组学分析
- Author:
Xinchao BAN
;
Man LI
;
Yanjun GU
;
Dan LOU
;
Xiuping WEI
;
Xiulan ZHAO
;
Baocun SUN
- Publication Type:Journal Article
- Keywords:
Melanoma;
Metastasis;
Differential gel electrophoresis;
Glycolytic enzymes;
B16-F10
- From:
Chinese Journal of Clinical Oncology
2010;37(5):246-249
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate differentially expressed protein profiles in B16-F10 grafted melanoma and its metastasis in the lung in order to identify molecular markers of melanoma metastasis. Methods: Differentially expressed proteins in B16-F10 grafted melanoma and its metastatic lesion in the lung were isolated and identified by fluorescence two-dimensional differential gel electrophoresis(2D-DIGE)coupled with matrix assisted laser desorption ionisation time-of-flight mass spectrometry(MALDI-TOF-MS).Some of identified proteins were further confirmed by Real-time PCR analysis. Results: High resolutional images of differential gel electrophoresis were obtained and 9 of 30 differentially expressed proteins (IRatiol≥2,P<0.01)were identified by MALDI-TOF-MS.The expression of Myoglobin(MB),vimentin(VIM),phosphoglycerate kinase 1(PGK1),Triosephosphate isomerase(TPI or TIM),heavy-chain binding protein(BiP),α-enolase,β-actin,γ-actin,and laminin-binding protein were up-regulated in the experimental group compared with the control group.These proteins were involved in the cytoskeletal formation,glycolysis and so on.Real-time PCR analysis showed up-regulation of mRNA expression of PGK1 and TPI in the experimental group(P=0.001 and 0.003),which was in consistent with the resuits of proteomic analysis. Conclusion: A variety of abnormally expressed proteins contribute to the metastasis of mice melanoma.Glycolytic enzymes PGK1 and TPI may be involved in this process.
