Effects of heparinase Ⅰ on syndcan-1 and extracelluar signal regulated kinase expression in human umbilical vein endothelial cells following hypoxia/reoxygenation
10.3969/j.issn.1673-8225.2010.11.024
- VernacularTitle:人脐静脉血管内皮细胞缺氧复氧损伤时黏结合蛋白多糖1及细胞外信号调节酶表达与肝素酶Ⅰ的调节
- Author:
Haiyan LUO
;
Changlin HU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(11):1995-1999
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Heparinase can induce syndecan-1 shedding from tumor cells and macrophage motion may correlate with biosynthesis regulation of heparan sulfate proteoglycan.OBJECTIVE:To investigate the effects of heparinase Ⅰ on syndecan-1 and extracelluar signal regulated kinase 2(ERK2)in human umbilical vein endothelial cells(HUVECS)with hypoxia/reoxygenation injury.METHODS:heparinase Ⅰ-precultured HUVECS were treated with hypoxia/reoxygenation.Immunohistochemistry staining,RT-PCR and western blot were applied to detect HUVECS syndecan-1 and ERK2 expression.RESULTS AND CONCLUSION:Expression of syndecan-1 and ERK2 was increased in HUVECS following hypoxia/reoxygenation treatment.Heparinase Ⅰ significantly upregulated the expression of syndecan-1 and ERK2 in HUVECS with hypoxia/reoxygenatiOn treatment.Syndecan-1 and ERK2 expression was positively related.Results show that syndecan-1 and ERK2 participate the pathophysiology of HUVECs hypoxia/reoxygenation injury Heparinase Ⅰ influences ERK2 expression by regulating syndecan-1.
