Construction of tissue-engineering heart valve scaffold with three cell detergents
10.3969/j.issn.1673-8225.2010.08.005
- VernacularTitle:构建组织工程瓣膜支架中3种去垢剂的比较
- Author:
Zhengqing LI
;
Jie MA
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(8):1349-1352
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: The method and effect of construction of tissue-engineering heart valve scaffold with cell detergents are different, and the method of detergent combining with the trypsase and nucleese is more suitable than others. OBJECTIVE: To investigate the effect of different detergents (sodium deexycholate, sodium dodecylsulfate, and triton) combined with trypsase on decellularized porcine heart valve and the influence on the accellular scaffold. METHODS: Porcine aortic valve leaflets were sterilized by antibiotics for 12 hours, maintained in the solution of the trysin and the EDTA for 12 hours, and treated with sodium deexycholate, sodium dodecylsulfate, and triton. Finally, the sample was dip in nucleese solution for 12 hours to remove endothelial cells and interstitial cells. HE staining was used to detect whether the endothelial cells were removed completely, Masson staining was used to evaluate damage level of collagen fiber and elastic fiber, and electronic scanning was used to observe the microstructure. RESULTS AND CONCLUSION: All the three detergents completely removed the endothelial ceils; however, the effect of sodium decxycholate on collagen fiber and elastic fiber was light, and then sodium dodecylsulfate and triton. This suggested that the method of DOA combining with the enzyme digestion was a suitable technique to construct tissue-engineering heart valve scaffold.
