The Effect of Herbal Compound for Reinforcing Kidney, Activating Blood and Arousing Consciousness on the Expression of NT-3 of the Cochlea in Gentamicin-induced Ototoxic Mice
- VernacularTitle:补肾活血通窍中药复方对庆大霉素耳中毒小鼠耳蜗NT-3表达的影响
- Author:
Qunzhen LI
;
Yongling SONG
;
Lin XU
;
Guangjie ZHU
;
Xia GAO
- Publication Type:Journal Article
- Keywords:
Chinese herbal medicine;
Compound;
Mice;
Cochlea;
Neurotrophic factor 3
- From:
Journal of Audiology and Speech Pathology
2010;18(2):160-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of herbal compound in reinforcing the kidney for activating blood and arousing consciousness(HCRAA) on the ABR threshold and the expression of neurotrophic factor 3(NT-3) of the cochlea of gentamicin (GM)-induced ototoxic mice.Methods 40 mice were randomly divided into normal group,model group,and high,middle and low concentration HCRAA groups.Normal group received no treatment.The model group and the HCRAA groups were intraperitoneally injected with GM 100mg/kg per day for consecutive 15 days.At the same time,the model group and the HCRAA groups respectively receiveded normal saline and high,middle and low concentration Chinese medical formula decoction at the same dosage by garage for consecutive 20 days.After the experiment,the mice were tested ABR.The expression of NT-3 of the cochlea in mice was detected by western blot.Results HCRAA at high and middle concentration reduced the GM elevated ABR threshold(P＜0.01),and increased the expression of NT-3 in the cochlea(P＜0.01).Conclusion HCRAA may effectively reduce the elevated ABR threshold induced by gentamycin by protecting GM damaged cochlear hair cell and neurons and increasing the expression of NT-3 in the cochlea.
