Expressions of Smad3 and transforming growth factor beta 1 in keloid,hypertrophic scars and normal skins:A 48:40:40 sample pathological detection
10.3969/j.issn.1673-8225.2010.11.007
- VernacularTitle:Smad3和转化生长因子β1在瘢痕疙瘩、增生性瘢痕及正常皮肤中的表达:48:40:40例标本病理检测
- Author:
Jiuling PANG
;
Zheng MA
;
Jun LIU
;
Aidong LIU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(11):1927-1930
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:Study regarding Smad3/transforming growth factor-β1(TGF-β1)signal transduction in pathological scars mainly focus on in vitro cultured fibroblasts,however,the correlation study was rare on keloid.The aim of this study is to detect the expressions of Smad3 and TGF-β1,and investigate their relationship in pathogenesis and development of pathological scars METHODS:Experimental samples were obtained frOm the patients who underwent burn and plastic surgery at the Department of Burn and Plastic Surgery,Workers'Hospital of Tangshan,Hebei Medical University,from June 2004 to June 2008,including 48 patients with Keloid,aged 16-52 years,and 40 patients with hypertrophic scars aged 18-56 years.Normal skins from additional 40 cases were served as controls The expressions of Smad3 and TGF-β1 protein in keloid,hypertrophic scars and normal skin were examined by flow cytometry.RESULTS:The expression of Smad3 and TGF-β1 were obviously greater in the experimental group than that of the control group (P＜0.05),but the difference between keloid and hypertrophic scars had no significance(P＞0.06).There was a positive correlation between Smad3 and TGF-β1 in keloid and hypertrophic scars(r=0 489 2,P=0.000 4:r=0.471 0,P=0.002 2).No notable correlation was found between Smad3 and TGF-β1 in normal skins(P=0.471 4).CONCLUSION:The expressions of Smad3 and TGF-β1 are up-regulated and the synergism of Smad3 and TGF-β1 may promote the development in pathological scars.
