Changes of glucocorticoid-induced osteonecrosis of the femoral head under mechanics load
10.3969/j.issn.1673-8225.2010.10.016
- VernacularTitle:力学载荷下大鼠激素性股骨头坏死的改变
- Author:
Zhigang WANG
;
Xinlong MA
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(11):1963-1966
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Femoral head necrosis induced by glucocorticoid is typical under mechanics load.OBJECTIVE:To create the rat models of the femoral head necrosis by long-term glucocorticoid treatment,and to Observe the changes of femoral head necrosis induced by glucocorticoid under mechanics load.METHODS:Wistar rats with 4-month old were randomly divided into the experimental and control groups.After the buttocks injection of dexamethasone 20mg/kg weakly for 8 weeks,rats in the experimental group were made to run on the running machine at a speed of 1 km/h to forme the hyper-weight model;at the same time those in the control group were with the normal physiological weight.Rats in both groups were sacrificed at weeks 2,4,6 and 8.The left legs were taken for the biomechanics test on the EnduraTEC ELF3200,the biggest vertical deformation and stiftness were calculated,the number of empty osteocyte lacunas was calculated,and the cumulative absorbance value was compared between two groups.RESULTS AND CONCLUSION:The femoral head presented with necrosis at 8 weeks in the experimental group.and the width of bone trabecula,and the stiffness of which were smaller than that of the control group.but the biggest vertical deformation was greater the concurrent control group(P<0.05).The Bcl-2 cumulative absorbance value of 2 groups had significant differences at 4 weeks(P<0.05),which was increased with time prolonged in the experimental group(P<0.05).The results demonstrated that:in glucocorticoid-induced osteonecrosis of the femoral head,mechanics Ioad induces the obviously osteocyte necrosis.more cartilage repair,and trabeculae rupture,which is considered as the direct reason of the necrosis of the femoral head The typical femoral head necrosis is presented.