Influence of beta nerve growth factor in vibrissa follicle bulge region on the burn wound healing of neonatal rats
10.3969/j.issn.1673-8225.2010.11.022
- VernacularTitle:毛囊隆突区β神经生长因子水平对乳鼠烫伤创面愈合的影响
- Author:
Jun ZENG
;
Dengbang CHEN
;
Lüxia DAI
;
Sen LIN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(11):1987-1991
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The surroundings stimulation,such as wound and chemical injury,will result in changes of hair follicle stem cells(HFSC).Up to date,few articles indicate the effecl of burn on HFSCs.OBJECTIVE:To investigate the changes of β-nerve growth factor(β-NGF)level in vibrissa follicle bulge region and its effect on the healing of neonatal rats.METHODS:A total of 30 neonatal rats were selected to prepare burn models by giving 90℃ boiled tap water for 3 seconds,and vibrissa follicle bulge was separated at 12,24,36,48 and 60 hours pOsloperatiVeIy.The remained 6 neonatal rats were served as controls.Total protein concentration was measured by Brandford method The expression of β-NGF was detected by ELISA.Meanwhile the tissues in each time points were collected for frozen section and immunohistochemistry.RESULTS AND CONCLUSION:Among the tested total protein.the expression of the β-NGF increased after 36 hours.reached a peak at 60 hours after burn.The identical changes were found in tissue slices.The image analysis demonstrated that the β-NGF protein detection was coincident with β-NGF expression in tissues During 12 hours to 60 hours after burn,the expression of the β-NGF in the bulge area peaked at 60 hours;simultaneously,the repairing effect of healing also reached a peak.Bulge area is a nest of HFSC,the enhanced expression of β-NGF may have some positive significance to the differentiation of the HFSC and tissue repair.
