Repairing rabbit femur bone defects by porous silk fibroin/hydroxyapatite combined with adipose-derived stromal cells
10.3969/j.issn.1673-8225.2010.08.003
- VernacularTitle:多孔型丝素蛋白/羟基磷灰石复合脂肪间充质干细胞修复兔股骨骨缺损
- Author:
Yongyi SHI
;
Genlin WANG
;
Huilin YANG
;
Shenzhou LU
;
Ya ZHANG
;
Xin CAI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(8):1341-1344
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Silk fibroin derived from silk had a good biocompatibility and biodegradation, which could be used for biomaterials to improve cell adhesion and growth abilities. OBJECTIVE: To investigate the efficacy of silk fibroin/hydroxyapatite (SF/HA) compounded of adipose-derived stromal cells (ADSCs) on repairing bone defects. METHODS: Adipose tissues were derived from epididymis of 2-month-old New Zealand rabbits and trypsogen-passaged to obtain ADSCs. The third-passage ADSCs at the concentration of 1×10/L were placed on SF/HA scaffold. Three hours later, the composite was cultured with DMEM culture media containing 1 μmol/L dexamethasone, 50 μmol/L vitamin C, and 10 mmol/L β-sodium glycerophosphate. Thirty-six rabbits were induced cancellated bone defect sizing 4.5 mm × 4.5 mm × 10 mm. The composite group was implanted with SF/HN/ADSCs scaffold, the simple group was implanted with SF/HA scaffold, but any treatment was employed in the blank control group. RESULTS AND CONCLUSION: At 12 weeks, general observation demonstrated that the bone defects were repaired entirely in composite group and partly in simple group. However, the bone defect was not repaired in the blank control group. X-ray and histological observation suggested that at 12 weeks the bone defects were repaired entirely in composite group and partly in simple group. The quantity of the newly formed bone in the composite group was significantly more than that in the simple group (P < 0.05). Repair showed no effect in the blank control group. SF/HA/ADSCs composite could successfully repair bone defects of a rabbit femur, and the effect was superior to SF/HA scaffold.
