Compatibility of osteoblasts on the polylactic acid-chitosan fiber/hydroxyapatite-calcium silicate composite scaffold material
10.3969/j.issn.1673-8225.2010.08.016
- VernacularTitle:聚乳酸-壳聚糖纤维/羟基磷灰石-硅酸钙复合支架材料的细胞相容性
- Author:
Li WEI
;
Dechao LI
;
Jing WANG
;
Xia HOU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(8):1397-1401
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Practice has proved that organic material and inorganic materials used alone are not ideal scaffold materials. Polylactic acid (PLA) possessing excellent biocompatibility, degradability and absorbability, PLAs composites will be one of the most important biocomposite in the 21~(st) century. OBJECTIVE: To observe the effects of PLA-chitosan fiber (CF)/hydrexyapatite-calcium silicate (HA-CS) on adhesion, proliferation and differentiation of osteoblasts. METHODS: The rat osteoblasts were obtained from the cranium of newborn Wistar rats within 24 hours, and primarily cultured using modified collagenase digestion. The cells were generated and their biological characteristic was examined by inverted phase-contrast microscope, hematoxylin-eosin staining, alkaline phosphatase (ALP) staining and mineralized nodules staining. Then the cells at passage 3 were co-cultured with PLA-CF, PLA-CF/CS and PLA-CF/HA-CS in vitro. At 3, 6 and 9 days of the culture, cell morphology was observed by inverted phase contrast microscopy. In addition, MTT assay and ALP activity test were used to observe the effects of three kinds of materials on cell differentiation and proliferation. RESULTS AND CONCLUSION: Osteoblasts attached on all three scaffold materials can adhere, grow, differentiate and proliferate. The effect of three materials on cell activity was PLA-CF/HA-CS>PLA-CF/CS>PLA-CF. The composite scaffold PLA-CF/HA-CS has a good compatibility, indicating that the material has a great potential for application in bone tissue engineering.
