The modified inoculation method for the preparation of rabbit VX2 hepatic carcinoma model and DSA imagining observation
- VernacularTitle:兔肝癌模型的改良接种及其DSA影像分析
- Author:
Yaopu WANG
;
Kangjian YANG
;
Siyuan ZHAO
;
Binyu ZHAO
;
Wei CAO
;
Chao LI
;
Qingfeng YANG
;
Zhimin WANG
;
Hongxin ZHANG
- Publication Type:Journal Article
- Keywords:
hepatic carcinoma;
rabbit VX2 transplanted tumor model;
digital subtract angiography
- From:Journal of Interventional Radiology
2010;19(3):214-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a suitable and stable metastatic rabbit VX2 liver tumor model for the use in experimental study,to discuss the successful rate of different tumor transplanting methods,and to analyze the digital subtract angiography(DSA)imagining features of the transplanted liver tumor.Methods Sixty male New Zealand white rabbits were randomly and equally divided into 3 groups with 20 rabbits in each group.For the rabbits of two groups,receiving injection methods and used as control groups,VX2 carcinoma particle(containing about 5×107 carcinoma cells) was inoculated into the left hepatic lobe through injection via hepatic artery or through direct injection with a syringe needle.For the rabbits in the retrofit group tumor tissue particle(containing about 106-108 carcinoma cells)was directly transplanted into liver through puncturing of the Glisson's capsule.The observation included the following two respects. (1)The tumor's survival rate of the three groups was evaluated.(2)The DSA imaging feature of the transplanted tumor was observed.Results The survival rate of the transplanted tumor in three groups was 7/20,10/20 and 19/20 respectively,with the survival rate of the retrofit group being the highest in 3 groups(P＜0.05).On DSA the transplanted tumors were rich in blood supplying.Conclusion For the establishment of rabbit VX2 liver carcinoma model,the direct transplantation of the tumor tissue particle is obviously superior to the injection method(direct injection or through hepatic arterial injection)in obtaining higher successful rate.This technique provides clinical and fundamental liver cancer studies with a reliable,stable and mature tumor animal model.
