Proteomic Screening of Serum Hepatocellular Carcinoma-associated Proteins by an Acetonitrile-pretreatment Method
- VernacularTitle:乙腈预处理血清寻找肝细胞癌发病相关的蛋白质
- Author:
Jianwen ZHANG
;
Wei LIU
;
Binsheng FU
;
Kunhua HU
;
Shaojun LIU
;
Hua LI
;
Qi ZHANG
;
Mingtao LI
;
Guihua CHEN
- Publication Type:Journal Article
- Keywords:
acetonitrile pretreatment;
hepatocellular carcinoma;
serum
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2010;31(2):288-292
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To apply an acetonitrile-pretreatment method for proteomic analysis of serum of patient with hepatocellular carcinoma(HCC),searching for the proteins that might be related to occurrence and development of HCC.[Methods]Serum samples were recruited from 12 patients with primary HCC and 12 health adult.Firsdy,the sera were pretreated with acetonitrile (ACN),and the high-abundance proteins were removed,then two-dimensional electrophoreaia(2-DE)was performed to screen the differentially expressed proteins between the patient with HCC and health control.Finally,the differentially expressed proteins in the 2-DE gel were identified by a MALDI-TOF/TOF mass spectrometry.[Results]After the sera were pretreated with 0%,20% and 30% ACN,2-DE analysis showed that the number of the protein spots in the gels were 532±96,623±102,and 674±123,respectively.By the proteomic analysis of the ACN-pretreatment sera of patient with HCC and health control,we found that tetraiodo-L-thyronine and proapolipoproteinin were up-regulated,vitamin D-binding protein precurser and transferrin were downregulated in the HCC serum.[Conclusion]Proteomic analysis of serum using the ACN-pretreatment method can improve the detection of proteins which were non-specifically binding to the albumin.Abnormal expression of tetraiodo-L-thyronine,proapolipoproteinin,vitamin D-binding protein precursor and transferrin in the serum are related to HCC.
