Selective Expansion of TCR V beta 3+CD4+ T Cells in Collagen-induced Arthritis in DBA/1 Mice.
- Author:
Jae Seon LEE
1
;
Mi La CHO
;
Jung Eun LEE
;
So Youn MIN
;
Chong Hyeon YOON
;
Wan Uk KIM
;
Jun Ki MIN
;
Sung Hwan PARK
;
Ho Youn KIM
Author Information
- Publication Type:In Vitro ; Original Article
- Keywords: TCRVbeta 3; antigen-specific T cell; type II collagen; collagen-induced arthritis
- MeSH: Animals; Arthritis; Arthritis, Experimental*; Autoimmune Diseases; Cell Proliferation; Collagen Type II; Cytokines; Humans; Immunization; Immunoglobulin G; Interleukin-10; Interleukin-17; Interleukin-4; Lymph Nodes; Mice*; Models, Animal; Receptors, Antigen, T-Cell; Rheumatic Fever; T-Lymphocytes*; Tumor Necrosis Factor-alpha
- From:Immune Network 2005;5(2):78-88
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Collagen-induced arthritis (CIA) in mice is animal model of autoimmune disease known as rheumatic arthritis in human. We investigated CII-specific CD4+ T cell receptor usage in CIA mice. METHODS: In CIA model, draining lymph node (dLN) CD4+ T cells and splenocytes at 3rd, 5th, 8th week, we investigated CII-specific T cell proliferation, production of IL-17, IFN-gamma, TNF-alpha, IL-4 and IL-10. And we also performed anti-CII IgG Ab measurements in serum level, TCRVbeta usage and T cell clonality with RT-PCR-SSCP analysis. Also, we performed proliferative response against CII when CII-specific T cell subset is deleted. RESULTS: CIA mice showed more increase in the serum level of anti-CII IgG than normal mice after induction of arthritis. And the level of anti-CII IgG2a in CIA mice was increased after 3rd week after primary immunization, while anti-CII IgG1 was decreased. Draining LN CD4+T cells have proliferated against CII stimulation at 3rd week after 1st immunization. CD4+T cells derived from dLN of CIA mice produced proinflammatory cytokine IFN-gamma, IL-17 etc. Draining LN CD4 T cells of CIA presented higher proportion of CD4+Vbeta +subsets compared to those of normal mice at 3rd week after 1st immunization, and they were increased in proportion by CII stimulation. Draining LN CD4+ T cells without TCRVbeta +/Vbeta 8.1/8.2+/Vbeta 10b+cells were not responsive against CII stimulation. But, CII-reactive response of TCRVbeta 3-/Vbeta 8.1/8.2-/Vbeta 10b- T cells was recovered when Vbeta 3+ T cells were added in culture. CONCLUSION: Our results indicate that CD4+Vbeta 3+ T cells cells are selectively expanded in dLN of CIA mice, and their recovery upon CII re-stimulation in vitro, as well as the production Th1-type cytokines, may play pivotal role in CIA pathogenesis.
