Expression and purification of soluble recombinant Hexastatin in E.coli
- VernacularTitle:重组人Hexastatin融合蛋白在大肠杆菌中的可溶性表达及其纯化
- Author:
Lei WEN
;
Naling SONG
;
Xin HE
;
Dezhi WANG
;
Qiren ZHAO
- Publication Type:Journal Article
- Keywords:
Hexastatin;
prokaryotic expression;
purification
- From:
Chinese Journal of Biochemical Pharmaceutics
2010;31(2):81-84
- CountryChina
- Language:Chinese
-
Abstract:
purpose To construct the expression vector of Hexastatin gene,to express and to purify the recombinant protein for further activity research.Methods The human Hexastatin gene was isolated by RT-PCR from EC9706 cells total RNA and cloned into pMDl8-T for sequencing.Then the Hexastatin gene was subcloned into pMAL-c4x expression vector and induced to express by IPTG.The recombinant fusion protein was purified with Amylose Resin Heads.Results RT-PCR product was about 687 bp and its sequence was the same as that of Hexastatin reported.The recombinant protein was expressed in E.coli BL21 with high level and the soluble protein accounted for 24.8% of the total bacterial protein,The purification of recombinant protein purified with Amylose Resin Heads reached more than 90%.Conclusion The cloning,expression and purification of human Hexastatin have laid a foundation for its anti-angiogenesis therapy for tumor.
