A Combination of PG490 and Lipopolysaccharide Induce Apoptosis through Activation of Casapase-3 and Down- regulation of cIAP1 and XIAP in Human Astroglioma Cell.
- Author:
Tae Jin LEE
1
;
Kyung Jin WOO
;
Jong Wook PARK
;
Taeg Kyu KWON
Author Information
- Publication Type:Original Article
- Keywords: LPS; PG490; apoptosis; astroglioma
- MeSH: Apoptosis*; Astrocytoma*; Central Nervous System; Cytochromes c; DNA Fragmentation; Glioma; Humans*
- From:Immune Network 2005;5(2):99-104
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Malignant gliomas are the most common primary tumors in the central nervous system. METHODS: We investigated the combined effect of PG490 and LPS on the induction of the apoptotic pathway in human astroglioma cells. RESULTS: Treatment of U87 cells with combination of 50 nM of PG490 and 50microgram/ml of LPS resulted in increased internucleosomal DNA fragmentation, cleavage of PLC-gamma1, and down- regulation of cIAP1 and XIAP. The combination of LPS and PG490 treatment-induced apoptosis is mediated through the activation of caspase, which is inhibited by the caspase inhibitor, z-VAD-fmk. Also, release of cytochrome c was found in PG490 and LPS- cotreated U87 cell. CONCLUSION: Taken together, combination of PG490 and LPS appears to be a potent inducer of apoptosis in astrogliaoma cells, and might have some benefit in the treatment of glioma patients.
