The Expression of Galectin-3, a Beta-Galactoside Binding Protein, in Dendritic Cells.
- Author:
Mi Hyoung KIM
1
;
Hong Gu JOO
Author Information
- Publication Type:Original Article
- Keywords: Dendritic cells; galectin-3; viability; proliferaion
- MeSH: Animals; Blotting, Western; Carrier Proteins*; Colony-Stimulating Factors; Dendritic Cells*; Flow Cytometry; Galectin 3*; Mice
- From:Immune Network 2005;5(2):105-109
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Dendritic cells (DCs) are the most potent APCs (antigen-presenting cells) and play a critical role in immune responses. Galectin-3 is a biological lectin with a beta-galactoside binding affinity. Recently, proteomic analysis revealed the presence of galectin-3 in the exosome of mature DCs. However, the expression and function of galectin-3 in DCs remains unclear yet. METHODS: We used bone marrow-derived DCs of mouse and showed the expression of galectin-3 in DCs by using flow cytometry analysis and Western blot analysis. RESULTS: Galectin-3 was determined as single band of 35 kDa in Western blot analysis. Flow cytometry analysis showed the major growth factor for DCs, granulocyte-macrophage colony stimulating factor (GM-CSF) and maturing agents, anti-CD40 monoclonal antibody (mAb) and lipopolysaccharide (LPS) consistently increased the intracellular expression of galectin-3 in DCs compared to medium alone. In addition, DCs treated with maturing agents did marginally express galectin-3 on their surface. CONCLUSION: This study suggests that galectin-3 in DCs may be regulated by critical factors for DC function.
