Effects of Endotoxin on Growth Activity and Gap Junction of NRK52E Cells
- VernacularTitle:内毒素对NRK52E细胞生长及细胞间缝隙连接功能的影响
- Author:
Jing WEI
;
Xiaoliang GAN
;
Chenfang LUO
;
Xiaoyun LI
;
Jinghui CHEN
;
Ziqing HEI
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
NRK52E cells;
gap junction;
cell growth
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2010;31(2):249-252
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]This study was designed to observe the effects of endotoxin(LPS)on the growth activity and gap junction(GJ)of NRK52E cells.[Methods]The NRK52E cells were divided into control group and LPS groups,and the NRK52E cells in LPS groups were treated with LPS 10 ng/mL,50 ng/mL,100 ng/mL,500 ng/mL,and 1 000 ng/mL for 24 h respectively.The NRK52E cells growth activity was measured through MTT method,and the function of gap junction of NRK52E cells was measured through the method of fluoroimmunoassay.The protein expression of connexin 43(Cx43)in control group,LPS 10 ng/mL group,and LPS 100 ng/mL group were also determined by Western blotting.[Results]①Compared with control group and LPS 10 ng/mL group,The NRK52E cells growth activity decreased significantly in LPS 50 ng/mL,100 ng/mL,500 ng/mL,and 1 000 ng/mL groups(P＜0.01).②Compared with control group,the function of GJ decreased significantly in LPS 100 ng/mL,500 ng/mL,and 1 000 ng/mL groups(P＜0.01).③There were negative correlations among the concentration of LPS and NRK52E cells growth activity and GJ function respectively(r=-0.941,-0.872,P＜0.01).④Compared with control group,the protein expression of Cx43 were decreased significantly in LPS 10 ng/mL and 100 ng/mL groups(P＜0.01).[Conclusions]LPS can inhibit the NRK52E cells growth activity in a dose-depend manner.GJ function is one of the mechanisms.
