Effect of different prone time/supine time ratios to pathology of mini-pig with acute lung injury

VernacularTitle:24h内不同俯/仰卧位通气时间比对急性肺损伤模型病理的影响
Author: Yanling WANG ; Qian XIA ; Ying WU
Publication Type:Journal Article
Keywords: Acute lung injury; Prone position; Mini-pig
From: Chinese Journal of Practical Nursing 2011;27(5):1-4
CountryChina
Language:Chinese
Abstract: Objective To compare the effects of different prone and supine position duration within 24-hour period on mini-pig acute lung injury (ALI)models. Methods Fifteen female mini-pigs,mean weight (30.2±3.2) kg, length (76.5±5.9) cm were anesthetized and ventilated. Hydrochloric acid (pH 1.0)was aspirated intratracheally to create ALI model. Mini-pigs were assigned randomly into three groups: p rone 8 h group, prone 8h then supine 16 h; 16 h group, prone 16 h then supine 8 h; 24 h group, prone 24 h continuously. Min-pigs were euthanasia at the end of the experiment to take out the lung, examine the tissue change and calculate the wet to dry weight ratio of lung (W/D). Sample was cut into slices and carried on hematoxylin and eosin (HE) stain which was intended to calculate injury score by semi ration score criteria. Results W/D, and injury score were lower in 16 h group and 24 h group than the 8 h group,whereas no significant differences among the three groups. Conclusions Prone position for 16 hours or longer could decrease oxygenation by decreasing inflammation in acute lung injury(ALI)animal models.