Isolation of human umbilical cord mesenchymal stem cells and differentiation into adipocytes and osteblasts
10.3969/j.issn.1673-8225.2010.14.005
- VernacularTitle:人脐带间充质干细胞分离培养及向脂肪与成骨细胞的分化
- Author:
Shaoqing HE
;
Zhenyu LUO
;
Qiuying LIU
;
Xiangrong ZHOU
;
Mingquan DENG
;
Xin LUO
;
Runsi YAO
;
Zhi GAO
;
Yifei WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(14):2492-2496
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Culture condition,isolation method and efficiency are different in reported human umbilical cord-derived mesenchymal stem cells,which lack of unified identification standards.Therefore,it is necessary to establish a high-efficiency and economical culture system for human umbilical cord-derived mesenchymal stem calls(hUCMSCs).OBJECTIVE:To isolate hUCMSCs and induced differentiate into adipocytes and osteblasts.METHODS:The hUCMSCs were isolated form human umbilical cord by tissue adherence and digested with collagenase.The morphology,proliferation and immunophenotype of the 3rd passage cells were analyzed,and then cells were induced to osteogenic and adipogenic differentiation in vitro.RESULTS AND CONCLUSION:The hUCMSCs isolated from human umbilical cord by tissue adherence and digested with collagenase could be cultured and proliferated in vitro.Flow cytometry analysis revealed that the hUCMSCs were positive for CD29 CD44,CD59,CD105,but were negative for CD40,CD86 and HLA-DR.These calls could be induced to differentiate into adipocytes and osteblasts under proper inducing conditions.The hUCMSCs retained the appearance and phenotype even after being expanded more than 40 passages in vitro.This confirmed that the existence of MSCs in human umbilical cord and they had the capacity of differentiating into adipocytes and osteblasts.
