Primary culture of spiral ganglion cells from cochlea of neonatal C57 mice
10.3969/j.issn.1673-8225.2010.14.030
- VernacularTitle:新生C57小鼠耳蜗螺旋神经节细胞的原代培养
- Author:
Tingkuo WANG
;
Hong SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(14):2617-2620
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:At present,there are differences in reporting culture condition of cochleal spiral ganglion cells(SGCs)in circle of primary cell culture.Individual method has poor repeatability,and is not beneficial for practical application.OBJECTIVE:To primary culture and evaluate SGCs of neonatal C57 mice.METHODS:Tissues from cochlear modiolus were acquired from neonatal C57 mice by microanatomy.SGCs from cochlear modiolus were cultured by trypsin digestion,differential velocity adherent technique combined with chemicals.Cell growth was observed by inverted phase contrast microscope and hematoxylin and eosin staining.Immunocytochemistry was employed to classify cell types.RESULTS AND CONCLUSION:After purification,the call body of SGCs from cochlear modiolus was ellipse or triangle,with slender processes in cytoplasm.Nuclei were positive for Nuen(stained brown).Cytoplasm and axon were positive for β3-Tubulin stained brown).These indicated that mouse SGCs were successfully cultured.
