Biological characteristics of human umbilical cord mesenchymal stem cells following cryopreservation

VernacularTitle:人脐带间充质干细胞冻存复苏后的生物学特征
Author: Youwei WANG ; Zhibo HAN ; Shulin YAN ; Aibin MAO ; Bin WANG ; Ding WANG ; Ke CHEN ; Zhongchao HAN
Publication Type:Journal Article
From: Chinese Journal of Tissue Engineering Research 2010;14(10):1729-1733
CountryChina
Language:Chinese
Abstract: BACKGROUND:An effective freezing-thawing technique is crucial for the clinical application of human umbilical cord mesenchymal stem cells(UC-MSCs).OBJECTIVE:To investigate biological characteristics of UC-MSCs after cryopreservation.METHODS:UC-MSCs were isolated from human umbilical cord and frozen in liquid nitrogen.The survival rate and the suppressive effect of γ-interferon(IFN-γ)of cryopreserved-thawed and fresh human UC-MSCs were compared.Furthermore,the multiple potentials and phenotype of UC-MSCs were estimated after cryopreservation.RESULTS AND CONCLUSION:There was no significant difference between cryopreserved-thawed and fresh human UC-MSCs on the survival rate and the suppressive effect of IFN-γ of peripheral blood mononuclear cells(PBMCs).After cryopreservation,human UC-MSCs had the potential differentiation and the phenotype of mesenchymal stem cells.