Screening of optimal embryonic time for in vitro separation and culture of Kunming mouse embryonic stem cells:Comparison among 2.5,3.5,and 4.5 pregnant days
10.3969/j.issn.1673-8225.2010.10.014
- VernacularTitle:体外分离培养昆明小鼠胚胎干细胞最佳胚龄的筛选:孕2.5 d与3.5 d及4.5 d比较
- Author:
Haifeng ZHANG
;
Wei SHAN
;
Shujian QIN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(10):1780-1784
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Genotype of Kunming mice was similar to human population,thus an establishment of embryonic stem cell line is beneficial for research of transgenic animal.However,the best time to collect embryo has been less reported yet.OBJECTIVE:To find the best time to collect embryos from Kunming mice.METHODS:The embryos were collected from mother mice of 2.5,3.5,and 4.5 pregnant days.Microscope was used to evaluate the growth condition of embryos,embryo attaching rate(A/C),inner cell mass(ICM)growing rate(I/C),embryonic stem cells (ESCs)clone growing rate(P1/C)and ESCs subclone growing rate(P2/C).The cells were then stained with alkaline phosphatase.RESULTS AND CONCLUSION:Most of the 2.5-pregnant-day embryos were 16-cell-phase embryos.The 3.5-pregnant-day embryos were morulas while the 4.5-pregnant-day embryos were blastulas.There were no significant differences in A/C,I/C,P1/C,P2/C between 2.5 and 3.5 pregnant days(P ＞ 0.05).The 4.5-pregnant-day indicators mentioned above were significantly greater than those two groups;therefore,4.5-pregnant-day embryos were the best source to culture,clone,isolate and passage ESCs.
