Adeno-associated virus vector-medicated green fluorescent protein transfected human amniotic epithelial cells in vitro
10.3969/j.issn.1673-8225.2010.10.028
- VernacularTitle:腺相关病毒介导绿色荧光蛋白基因体外转染人羊膜上皮细胞
- Author:
Hongwu LUO
;
Xiangjun HUANG
;
Xunyang LIU
;
Feizhou HUANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(10):1843-1846
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Human amniotic epithelial cells(AECs)are easy to obtain and can function as ideal seed cells for cell transplantation and tissue repair.Currently,marking and tracing of human AECs remains rarely reported.OBJECTIVE:To explore the efficiency of adeno-associated virus(AAV)vector-medicated green fluorescent protein(GFP)on in vitro cultured human AECs transfection.METHODS:Human amnion samples were harvested and trypsinized to isolate human AECs.The AECs were transfected with AAV-GFP,and the transfection efficiency was detected.RESULTS AND CONCLUSION:Human AECs were successfully primary cultured and passaged in vitro.AAV-GFP-transfected AECs stably and highly expressed GFP,with a transfection efficiency of 58%.
