Schwann cells purification by four different methods in vitro
10.3969/j.issn.1673-8225.2010.10.039
- VernacularTitle:体外纯化乳鼠许旺细胞的4种方法比较
- Author:
Gang CHEN
;
Caihong YANG
;
Linqiang TIAN
;
Fengjin GUO
;
Anmin CHEN
;
Kai SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2010;14(10):1892-1896
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Schwann cell is one of the major seed cells In peripheral nervous system and plays an important role in neural injury and neural disease.However,the source of Schwann cells is limited.And the purity of Schwann cells is affected due to the pollution of fibroblasts.Many purified methods have been proposed,but every one has its defect to satisfy the clinical demand.OBJECTIVE:To compare the differences among differential adhesion purified method,cold jet purified method,immunomagnetic beads selection purified method and G418 selection purified method to purify Schwann cells of neonatal rat in vitro.METHODS:Bilateral sciatic nerves of SD rats were harvested under sterile condition.Schwann cells were purified respectively using differential adhesion purified method,cold jet purified method,immunomagnetic beads selection purified method and G418 selection purified method.Cell viability was compared,and cell purity was determined by immunohistochemistry.RESULTS AND CONCLUSION:The purity of Schwann cells separated by differential adhesion method was low,but the viability was fair.The purity and viability of cells following cold jet method immunomagnetic beads selection method was high.The purity of cells separated by immunomagnetic beads selection methods was similar to that of cold jet method immunomagnetic beads selection method,but the cell viability was worse.The cell viability following G418 selection method was bad,but the purity was high.
