In Vivo Kinetics and Biodistribution of a Hantaan Virus DNA Vaccine after Intramuscular Injection in Mice
10.1007/s12250-010-3103-7
- Author:
Si WANG
;
Qing NIE
;
Lanyan ZHENG
;
Jun HU
;
Enjie LUO
- Publication Type:Journal Article
- Keywords:
Hantaa virus;
DNA vaccine;
Intramuscular injection;
Immunologic memory;
Nucleocapsid protein
- From:
Virologica Sinica
2010;25(3):177-182
- CountryChina
- Language:Chinese
-
Abstract:
To study the kinetics in vivo of a Hantaan virus DNA vaccine, we constructed a fusion DNA vaccine,pEGFP/S, by cloning the S segment of Hantavirus into the vector, pEGFP-C1, which encodes Green fluorescent protein EGFP. In this report, we provide evidence that pEGFP/S was distributed and persistently expressed for more than 60 days in several organs after inoculation. Our findings suggest that the persistent immune responses induced by a Hantaan virus DNA vaccine are likely due to the plasmid pEGFP/S deposited in vivo, which acts as a booster immunization.
